Diversidad genética del jengibre (Zingiber officinale Roscoe.) A nivel molecular
Avances de la última década
DOI:
https://doi.org/10.18041/entramado.2015v11n2.22239%20Palabras clave:
Marcadores moleculares, recursos fitogenéticos, mejoramiento genético, relaciones filogenéticas, Zingiber officinaleResumen
El jengibre (Zingiber officinale) es una especie medicinal, aromática y condimentaria que se cultiva principalmente en regiones tropicales y subtropicales del mundo. Por tratarse de una especie de propagación vegetativa, su variabilidad genética tiende a ser baja; sin embargo es posible hallar una amplia variabilidad genética principalmente en la China e India, considerándose actualmente como el principal centro de origen y diversidad de esta especie. Conocer y caracterizar la diversidad genética del jengibre es una tarea de vital importancia para fomentar programas de conservación ex situ o in vitro que ayuden a evitar la erosión genética de esta especie, así como para direccionar correctamente estrategias de mejoramiento genético. Marcadores moleculares han sido ampliamente usados en los últimos años con la finalidad de estudiar la variabilidad genética del jengibre, directamente a nivel del ADN. En este artículo, los avances en la investigación en diversidad genética del jengibre, usando marcadores moleculares, son revisados. Esto con la finalidad de hacer un análisis sobre sus implicaciones para la conservación y el mejoramiento genético de la especie.
Descargas
Referencias
2. AGARWAL, Milee; SHRIVASTAVA, Neeta; PADH, Harish. Advances in molecular marker techniques and their applications in plant sciences. In: Plant Cell Reports. April, 2008. vol. 27, no. 4, p. 617–31.
3. AHMAD, Dawood; KIKUCHI, Akira; JATOI, Shakeel Ahmad; MIMU- RA, M; WATANABE, Kasuo N. Genetic variation of chloroplast DNA in Zingiberaceae taxa from Myanmar assessed by PCR-restriction fragment length polymorphism analysis. In: Annals of Applied Biology. April, 2009. vol. 155, no. 1, p. 91–101.
4. ALI, Badreldin H; BLUNDEN, Gerald; TANIRA, Musbah O; NEMMAR, Abderrahim. Some phytochemical, pharmacological and toxicological properties of ginger ( Zingiber officinale Roscoe): a review of recent research. In: Food and Chemical Toxicology. September, 2008, vol. 46, no. 2, p. 409–20.
5. ARAGAW, Momina; ALAMEREW, Sentayehu; MICHAEL, Girma; TES- FAYE, Abush. Variability of ginger ( Zingiber officinale Rosc.) accesions for morphological and some quality traits in Etiopia. In: International Journal of Agricultural Research. 2011. vol. 6, no. 6 p. 444-457.
6. ASHRAF, Kamran; AHMAD, Altaf; CHAUDHARY, Anis; MUJEEB, Mohd; AHMAD, Sayeed; AMIR, Mohd; MALLICK, N. Genetic diversity analy- sis of Zingiber officinale Roscoe by RAPD collected from subconti- nent of India. In: Saudi Journal of Biological Sciences. April 2014. vol. 21, no. 2, p. 159–165.
7. BALIGA, Manheshwar S; HANIADKA, Raghavendra; PEREIRA, Mani- sha M; D’SOUZA, Jason J; PALLATY, Princy L; BHAT, Harshith P; PO- PURI, Sandhya. Update on the chemopreventive effects of ginger and its phytochemicals. In: Critical Reviews in Food Science and Nutrition. June, 2011. vol. 51, no. 6, p. 499–523.
8. BOSSETI, Fátima; ZUCCHI, Maria I; PINHEIRO, Jose B. Molecular and morphological diversity in Japanese rice germplasm. In: Plant Genetic Resources: characterization and utilization. July, 2011. vol. 9, no. 2, p. 229–232.
9. BUA-IN, Saowaluck; PAISOOKSANTIVATANA, Yingyong. Study of clonally propagated cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) and its relation of wild Zingiber species from Thailand revealed by RAPD markers. In: Genetic Resources and Crop Evolu- tion. March, 2010. vol. 57, no. 3, p. 405–414.
10. BUTT, Masood S; SULTAN, M. Tauseef. Ginger and its health claims: molecular aspects. In: Critical Reviews in Food Science and Nutrition. April, 2011. vol. 51, no. 5, p. 383–393.
11. CHANDRASEKAR, Arumugam; RIJU, Aikkal; SITHARA, Kandiyl; ANOOP, Sahadevan; EAPEN, Santhosh J. Identification of single nu- cleotide polymorphism in ginger using expressed sequence tags. In: Bioinformation. September, 2009. vol.4, no. 3, p. 119–122.
12. CHAVAN, Preeti; WARUDE, Dnyaneshwar; JOSHI, Kalpana; PA- TWARDHAN, Bhushan. Development of SCAR (sequence-characte- rized amplified region) markers as a complementary tool for identi- fication of ginger (Zingiber officinale Roscoe) from crude drugs and multicomponent formulations. In: Biotechnology and Applied Bioche- mistry. December, 2010. vol. 50, no.1, p.61-69.
13. CHAVES, Francisco Celio M; FIGUEIRA, Glyn M; PRAL, Yves M; CRA- VEIRO, Esmeraldino R; VAZ, Ana Paula A. Avaliação agronômica e ca- racterização química de acessos de gengibre (Zingiber officinale ) nas condições de Manaus, AM. En: Horticultura Brasileira. Julio, 2012 vol. 30, no. 2, p. 5805–5809.
14. CHONGTHAM, T; CHATTERJEE, R; HNAMTE, V; CHATTOPADH- YAY, P.K; KHAN, S.A. Ginger (Zingiber officinale Rosc) germplasm evaluation for yield and quality in south-ern West Bengal. In: Journal of Spices and Aromatic Crops. January, 2013. vol. 22, no. 1, p. 88–90.
15. DAS, Abhijit; KESARI, V; SATYANARAYANA, V. Madurai; PARIDA, Ajay; MITRA, Susmita; RANGAN, Latha. Genetic diversity in ecotypes of the scarce wild medicinal crop Zingiber moran revealed by ISSR and AFLP marker analysis and chromosome number assessment. In:Plant Biosystems. June, 2013. Vol. 149, no. 1, p. 111–120.
16. DAS, Premananda; RAI, S; DAS, A.B. Cytomorphological barriers in seed set of cultivated ginger ( Zingiber officinale Rosc.). In: Iranian Journal of Botany. 1999. vol. 8, no.1, p.119–129.
17. DHAMAYANTHI, K.P.M; SASIKUMAR, Bhaskaran; REMASHREE, Azhimala B. Reproductive biology and incompatibility studies in gin- ger ( Zingiber officinale Rosc). In: Phytomorphology. April, 2003. vol. 53, no. 2, p. 123–131.
18. DING, S.H; AN, K.J; ZHAO, C.P; LI, Y; GUO, Yingjie H; WANG, Z.F. Effect of drying methods on volatiles of Chinese ginger ( Zingiber offi- cinale Roscoe). In: Food and Bioproducts Processing. July, 2012. vol. 90, no. 3, p. 515–524.
19. FAOSTAT, 2015. Estadísticas agrícolas mundiales. (Citado 16 de Abril de 2015). Disponible en internet: <http://www.faostat.fao.org>. 20. GEIGER, James L. The essential oil of ginger and anaesthesia. In: Inter- national Journal of Aromatherapy. September, 2005. vol. 15, no. 1, p. 7–14.
21. GHOSH, Subhabrata; MAJUMDER, P.B; MANDI, Swati S. Species-spe- cific AFLP markers for identification of Zingiber officinale , Z. mon- tanum and Z. zerumbet (Zingiberaceae). In: Genetics and Molecular Research. February, 2011. vol. 10, no.1, p. 218–229.
22. GHOSH, Subhabrata; MANDI, Swati S. Study of Genetic Variation among Some Wild Landraces of Zingiber officinale Roscoe Corre- lated with Their Antioxidant Potential Status. In: Journal of Molecular Biology Research. December, 2011. vol.1, no.1, p. 77–87.
23. HANGELBROEK, H.H; OUBORG, N.J; SANTAMARIA, Luis; SCHWENK, S. Clonal diversity and structure within a population of the pondweed Potamogeton pectinatus foraged by Bewick’s swans. In: Molecular Ecology. October, 2002. vol. 11, no. 10, p. 2137–2150.
24. HARISARANRAJ, R; SURESH, K; SARAVANABABU, S. DNA Finger Printing Analysis among eight varieties of Zingiber officinale Rosc. By Using RAPD Markers. In: Global Journal of Molecular Sciences. June, 2009. vol. 4, no. 2, p. 103–107.
25. HASSANPOURAGHDAM, Mohammad Bagher; AAZAMI, Moham- mad Ali; SHALAMZARI, Mohammad Saf; BANEH, H. Doulati. Essential oil composition of Zingiber officinale Rosc. rhizome from the Iranian herb market. In: chemija. 2011. vol. 22, no. 1, p. 56–59.
26. ISLAM, K.M; ISLAM, Aminul K.M.A; RASUL, Mohammad G; SULTA- NA, N; MIAN, M.A.K. Genetic variability and character association in ginger ( Zingiber officinale Rosc.). In: Annal of Bangladesh Agriculture. 2008. vol. 12, no.1, p. 69-76.
27. JAN, Hikmat Ullah; RABBANI, Malik Ashiq; SHINWARI, Zabta Khan. Assessment of genetic diversity of indigenous turmeric (Curcuma longa L .) germplasm from Pakistan using RAPD markers. In: Journal of Medicinal Plants. March, 2011.vol. 5, no. p. 823–830.
28. JATOI, Shakeel Ahmad; KIKUCHI, Akira; MIMURA, Makiko; SAN- SAN-Yi; WATANABE, Kazuo N. Relationships of Zingiber species, and genetic variability assessment in ginger ( Zingiber officinale ) ac- cessions from ex-situ genebank, on-farm and rural markets. In: Bree- ding Science. June, 2008. vol. 58, no. 3, p. 261–270.
29. JATOI, Shakeel Ahmad; KIKUCHI, Akira; SAN-SAN-Yi; NAING, Khau Wai; YAMANAKA, Shinsuke.; WATANABE, Junko A; WATANABE, Kazuo N. Use of Rice SSR Markers as RAPD Markers for Genetic Diversity Analysis in Zingiberaceae. In: Breeding Science. June, 2006. vol. 56, no. 2, p. 107–111.
30. JATOI, Shakeel Ahmad; KIKUCHI, Akira; AHMAD, Dawood; WATA- NABE, Kazuo N. Characterization of the genetic structure of mango ginger (Curcuma amada Roxb.) from Myanmar in farm and gene- bank collection by the neutral and functional genomic markers. In: Electronic Journal of Biotechnology. November, 2010. vol. 13, no. 6, p. 1 - 11.
31. JATOI, Shakeel Ahmad; WATANABE, Kazuo N. Diversity analysis and relationships among ginger landraces. In: Pakistan Journal of Botany. 2013. vol. 45, no. 4, p. 1203–1214.
32. JIANG, Hongliag; SÓLYOM, Aniko M; TIMMERMANN, Barbara N; GANG, David R. Characterization of gingerol-related compounds in ginger rhizome ( Zingiber officinale Rosc.) by high-performance liquid chromatography/electrospray ionization mass spectrometry. In: Rapid Communications in Mass Spectrometry. October, 2005. vol. 19, no. 20, p. 2957–2964.
33. JIANG, Hongliag; XIE, Zhengzhi; KOO, Hyun Jo; McLAUGHLIN, Ste- ven P; TIMMERMANN Barbara N; GANG, David R. Metabolic profi- ling and phylogenetic analysis of medicinal Zingiber species: Tools for authentication of ginger ( Zingiber officinale Rosc.). In: Phytochemistry. August, 2006. vol. 67, no. 15, p. 1673–1685.
34. KALIA, Rajwant K; RAI, Manoj K; KALIA, Sanjay; SINGH, Rohtas; DHAWAN, A. K. Microsatellite markers: an overview of the recent progress in plants. In: Euphytica. February, 2011. vol. 177, no. 3, p. 309– 334.
35. KAR, Dattatreya; PATTANAIK, Pratap Keshari; ACHARYA, Laxmikan- ta; PANDA, Manoj Kumar; SATHAPATHY, Kamalakanta; KUANAR, Ananya; MISHRA, Budahdeva. Assessment of genetic diversity among some elite cultivars of ginger ( Zingiber officinale Rosc.) using isozyme and protein markers. In: Brazilian Journal of Botany. December, 2014. vol. 37, no. 4, p. 469–479.
36. KAVITHA, P.G; KIRAN, A.G; DINESH, Raj R; SABU, M; THOMAS, George. Amplified fragment length polymorphism analyses unravel a striking difference in the intraspecific genetic diversity of four species of genus Zingiber Boehm. From the Western Ghats, South India. In: Current Science. January, 2010. vol. 98, no. 2, p. 242–247.
37. KAVITHA, P.G; THOMAS, George. Population genetic structure of the clonal plant Zingiber zerumbet (L.) Smith (Zingiberaceae), a wild relative of cultivated ginger, and its response to Pythium. In: Euphytica. March, 2008. vol. 160, no. 1, p. 89–100.
38. KIZHAKKAYIL, Jaleel; SASIKUMAR, Bhaskaran. Variabilty for quality traits in a global germplasm collection of ginger ( Zingiber officinale R). In: Current Trends Biotechnology and Pharmacy. July, 2009. vol. 3, no. 3, p. 254–257.
39. KIZHAKKAYIL, Jaleel; SASIKUMAR, Bhaskaran. Genetic diversity analysis of ginger ( Zingiber officinale Rosc.) germplasm based on RAPD and ISSR markers. In: Scientia Horticulturae. May, 2010. vol. 125, no. 1, p. 73–76.
40. KIZHAKKAYIL, Jaleel; SASIKUMAR, Bhaskaran. Diversity, characteri- zation and utilization of ginger: a review. In: Plant Genetic Resources. August, 2011. vol. 9, no. 3, p. 464–477.
41. KLADMOOK, Maytinee; CHIDCHENCHEY, Sopida; KEERATINI- JAKAL, Vichien. Assessment of genetic diversity in cassumunar ginger (Zingiber cassumunar Roxb.) in Thailand using AFLP markers. In: Bre- eding Science. December, 2010. vol. 60, no. 4, p. 412–418.
42. KUBRA, I. Rahath; MOHAN RAO, L. Jagan. M. An Impression on Cu- rrent Developments in the Technology, Chemistry, and Biological Ac- tivities of Ginger ( Zingiber officinale Roscoe). In: Critical Reviews in Food Science and Nutrition. July, 2012. vol. 52, no. 8, p. 651–688.
43. KUN-HUAI, Wei; JIAN-HUA, Miao; HE-PING, Huang; SHAN-LI, Gao. Generation of autotetraploid plant of ginger ( Zingiber officinale Rosc.) and its quality evaluation. In: Pharmacognozy Magazine. Jul-Sep, 2011. vol.7, no. 27, p. 200–206.
44. LEE, Sok-Young; FAI, Wan Kah; ZAKARIA, Myriana; IBRAHIM, Hali- jah; OTHMAN, RofinaYasmin; GWAG, Jae-Gyun; RAO, V. Ramanatha; PARK, Yong-Jin. Characterization of polymorphic microsatellite mar- kers, isolated from ginger ( Zingiber officinale Rosc.). In: Molecular Ecology Notes. November, 2007. vol.7, no.6, p.1009–1011.
45. LINCY, Adinkudikuyya; JAYARAJAN, K; SASIKUMAR, Bhaskaran. Rela- tionship between vegetative and rhizome characters and final rhizo- me yield in micropropagated ginger plants ( Zingiber officinale Rosc.) over two generations. In: Scientia Horticulturae. September, 2008. vol. 118, no. 1, p. 70–73.
46. MAHDI, Harith Jameel; ANDAYANI, Retno; AZIZ, Ishak. Determina- tion of phylogenetic and molecular characteristics of three Malaysian Ginger Cultivars ( Zingiber officinale Roscoe) usinng microsatellite DNA. In: Tropical Life Sciences Research. December, 2013. vol. 24, no. 2, p. 65–76.
47. MENON, A. Nirmala. Chemical composition of the essential oil from leaves and roots of ginger ( Zingiber officinale ) from Kerala. In: Journal of Medicinal and Aromatic Plant Sciences. April-Jun, 2007. vol. 29, no. 2, p. 78–80.
48. MOHANTY, Sujata; PARIDA, Reena; KUANAR, Ananya; SAHOO, S; NAYAK, Sanghamitra. Evaluation of genetic fidelity of in vitro propa- gated shampoo ginger (Zingiber zerumbet Smith) using DNA based markers. In: Journal of Medicinal Plants Research. April, 2012. vol. 6, no. 16, p. 3143–3147.
49. NAMPOOTHIRI, Suresh V; VENUGOPALAN, V.V; JOY, Baena; SREE- KUMAR, M.M; MENON, A. Nirmala. Comparison of Essential oil Composition of Three Ginger Cultivars from Sub Himalayan Region. In: Asian Pacific Journal of Tropical Biomedicine. December, 2012. vol. 2, no. 3, p. S1347–S1350.
50. NAYAK, Sanghamitra; NAIK, Pradeep K; ACHARYA, Laxmikanta; Mukherjee, Arup K; Panda, Pratap C; Das, Premananda. Assessment of Genetic Diversity among 16 Promising Cultivars of Ginger Using Cytological and Molecular Markers. In: Zeitschrift fur Naturfors- chung. June, 2005. vol. 60c, no. 5-6, p. 485–492.
51. PADALIA, Rajendra Chandra; VERMA, Ram Swarop; SAH, A.N; KARKI, N; SUNDARESAN, V; SAKIA, D. Leaf and rhizome oil composition of Zingiber officinale Roscoe and their antibacterial and antioxidant activities. In: Asian Journal of traditional medicines. 2011. vol. 6, no. 2, p. 73–82.
52. PALAI, Siddharth Kumar; ROUT, Gyana Ranjat. Identification and ge- netic variation among eight varieties of ginger by using random ampli- fied polymorphic DNA markers. In: Plant Biotechnology. December, 2007. vol. 24, no. 4, p. 417–420.
53. PANDOTRA, Pankaj; GUPTA, Ajai P; GHANDIRAM; HUSIAN, Mohd K; GUPTA, Suphla. Genetic and chemo-divergence in eighteen core collection of Zingiber officinale from North-West Himalayas. In: Scientia Horticulturae. August, 2013. vol. 160, p. 283–291.
54. PANDOTRA, Pankaj; GUPTA, Ajai P; HUSAIN, Mohd K; GHANDI- RAM; GUPTA, Suphla. Evaluation of genetic diversity and chemical profile of ginger cultivars in north-western Himalayas. In: Biochemical Systematics and Ecology. June, 2013. vol. 48, p. 281–287.
55. PANDOTRA, Pankaj; GUPTA, Ajai P; KHAN, Saima; RAM, Gandhi; GUPTA, Suphla. A comparative assessment of ISSR, RAPD, IRAP, & RE- MAP molecular markers in Zingiber officinale germplasm characteri- zation. In: Scientia Horticulturae. October, 2015. vol. 194, p. 201–207.
56. PANDOTRA, Pankaj; HUSAIN, Mohd Kashif; RAM, Gandhi; GUPTA, Suphla.; GUPTA, Ajai Prakash. Retrotransposon based genetic status of North-West Himalayan Zingiber officinale revealed high heteroge- neity. In: Journal of Plant Biochemistry and Biotechnology. April, 2014. vol. 23, no. 2, p. 211–216.
57. PAWAR, Nilesh; PAI, Sandeep; NIMBALKAR, Mansingraj; KOLAR, Fir- dose; DIXIT, Ghansham. Induction of chlorophyll mutants in Zingiber officinale roscoe by Gamma Rays and EMS. In: Emirates Journal of Food and Agriculture. October, 2010. vol. 22, no. 5, p. 406–411.
58. PETER, K.V; RAVINDRAN, P; NIRMAL BABU, K; DIVAKARAN, M. HORTICULTURE (Vegetables , Tubers & Spice Crops ). Kerala (India), Octubre, 2007. (Citado 16 de Abril de 2015). Disponible en internet en: http://nsdl.niscair.res.in/jspui/bitstream/123456789/471/1/revi- sed%20Breeding%20of%20spices.pdf.
59. PRASATH, D; SAJI, K; SASIKUMAR, Bhaskaran; KRISHNAMOORTHY, B; ANANDARAJ, M. Genetic diversity and conservation in major spi- ces. In: National Symposium on Spices and Aromatic: exploting spi- ces production potential of the Deccan region (4: 8-10, December: Dharwad, Karnataka). Souvenir and Abstracts. Kerala, India.: Indian Society for Spices, 2011. p.41–57.
60. PREM, Jithin; KIZHAKKAYIL, Jaleel; THOMAS, Elizabeth; DHANYA, K; SYAMKUMAR, S; SASIKUMAR, Bhaskaran. Molecular characteri- zation of primitive, elite and exotic ginger genotypes to protect the biowealth of elite ginger accessions. In: Journal of Spices and Aromatic Crops. 2008. vol.17, no. 2, p. 85–90, 2008.
61. RAMACHANDRAN, Kishore. Poliploidy induced in ginger by colchi- cine treatment. In: Current Science. March, 1982. Vol. 5, no. 6, p.288- 289.
62. RASHID, Kamaludin; DARAN, Abu Bakar; NEZHADAHMADI, Arash; ZAINOLDIN, Khairul Hazmi; AZHAR, Shamrul; EFZUENI, Shahril. The effect of using gamma rays on morphological characteristics of ginger ( Zingiber officinale ) plants. In: Life Science Journal. February, 2013. vol. 10, no. 1, p. 1538–1544.
63. RAVINDRAN, P. N; NIRMAL BABU, K; SHIVA, K.N. Botany and Crop Improvement of Ginger. In: Ginger The Genus Zingiber. 1 ed. Washin- gton: P.N. Ravindran; K. Nirmal Babu. 2005. p.15-33.
64. RAVINDRAN, P.N; SASIKUMAR, Bhaskaran; GEORGE, Johnson K; RATNAMBAL, M.J; NIRMAL BABU, K; ZACHARIAH, John T; NAIR, R.R. Genetic resources of ginger (Zingiber ofticinale Rose.) and its conservation in India. In: Plant Genetic Resources Newsletter. 1994. no. 98, p. 1–5.
65. RENE, Yonn Ai; RASHID, Kamaludin; TAJUDIN, Anas; ZAINOLDIN, Khairul Hazmi; DARAN, Abu Bakar; NEZHADAHMADI, Arash; GO- LAM, Faruq. The contribution of muslim scientists in botanical scien- ce: studies on the using of gamma rays for ginger plants (Zingiber officinale ). In: Stem Cell. December, 2014. vol. 5, no. 4, p. 88–94.
66. SAJEEV, S; ROY, A. R; IANGRAI, B; PATTANAYAK, A; DEKA, B.C. Ge- netic diversity analysis in the traditional and improved ginger (Zingi- ber officinale Rosc.) clones cultivated in North-East India. In: Scientia Horticulturae. April, 2011. vol.128, no. 3, p. 182–188.
67. SALMON, Collen N.A; BAILEY-SHAW, Yvonne A; HIBBERT, Sheridan; GREEN, Cheryl; SMITH, Ann M; WILLIAMS, Lawrence A.D. Characte- risation of cultivars of Jamaican ginger ( Zingiber officinale Roscoe) by HPTLC and HPLC. In: Food Chemistry. April, 2012. vol. 131, no. 4, p. 1517–1522.
68. SANWAL, Satish K; RAI, N; SINGH, Jagdish; BURAGOHAIN, Juri. An- tioxidant phytochemicals and gingerol content in diploid and tetra- ploid clones of ginger ( Zingiber officinale Roscoe). In: Scientia Horti- culturae. March, 2010. vol. 124, no. 2, p. 280–285.
69. SASIDHARAN, Indu; VENUGOPAL, V.V; MENON, Nirmala A. Essen- tial oil composition of two unique ginger (Zingiber officinale Roscoe) cultivars from Sikkim. In: Natural Product Research. October, 2012. vol. 26, no. 19, p. 1759–1764.
70. SASIKUMAR, Bhascaran; GEORGE, Johnson K; RAVINDRAN, P.N. IISR varada- a new high yielding ginger (Zingiber officinale Rosc.) va- riety. In: Journal of Spices and Aromatic Crops. June, 1996. vol. 5, no. 1, p. 34–40.
71. SASIKUMAR, Bhascaran; SAJI, K.V; ANTONY, A; GEORGE, Johnson K; ZACHARIAH, T. J; EAPEN, S. J. IISR Mahima and IISR Rejatha - two high yielding and high quality ginger ( Zingiber officinale Rosc.) varie- ties. In: Journal of Spices and Aromatic Crops. June, 2003. vol. 12, no. 1, p. 34–37.
72. SCHLÖTTERER, Christian. The evolution of molecular markers - just a matter of fashion?. In: Nature reviews Genetics. January, 2004. vol. 5, p. 63–69.
73. SHARMA, Gurumayum Jitendra; PUKHRAMBAM, Chirangini; KI- SHOR, Rajkumar. Gingers of Manipur: diversity and potentials as bio- resources. In: Genetic Resources and Crop Evolution. June, 2011. vol. 58, no. 5, p. 753–767.
74. SHIRIN, Adel; Prakash, Jamuna. Chemical composition and antioxidant properties of ginger root ( Zingiber officinale ). In: Journal of Medicinal Plants Research. December, 2010. vol. 4, no. 24, p. 2674–2679.
75. SHUKLA, Yogeshwer; SINGH, Madhulika. Cancer preventive proper- ties of ginger: a brief review. In: Food and chemical toxicology. May, 2007. vol. 45, no. 5, p. 683–690.
76. SINGH, Chingakham B; NONGALLEIMA, K; BROJENDROSINGH, S; NINGOMBAM, Swapana; LOKENDRAJIT, N; SINGH, L.W. Biologi- cal and chemical properties of Zingiber zerumbet Smith: a review. In: Phytochemistry Reviews. March, 2012. vol. 11, no. 1, p. 113–125.
77. SINGH, Gurdip; KAPOOR, I.P.S; SINGH, Prathiba; HELUANI, Carola S; LAMPASONA, Marina P; CATALAN, Cesar A.N. Chemistry, an- tioxidant and antimicrobial investigations on essential oil and oleore- sins of Zingiber officinale . In: Food and Chemical Toxicology. October, 2008. vol. 46, no. 10, p. 3295–3302.
78. SINGH, Thingbaijam Dikash; DEVI, Khumallambal Devala; SHAGOL- SEM, Brojendro Singh; SINGH, Chingakham Brajakishor; DEVI, Huin- drom Sunitibila. Assessment of Genetic Stability in Traditional Ginger Cultivated in Manipur, India Based on Molecular and Chemical Mar- kers. In: Analytical Letters. November, 2013. vol. 46, no. 18, p. 2941– 2953.
79. SMITH, M.K; HAMILL, S.D; GOGEL, B. J; SEVERN-ELLIS, A. A. Ginger ( Zingiber officinale) autotetraploids with improved processing quality produced by an in vitro colchicine treatment. In: Australian Journal of Experimental Agriculture. November, 2004. vol. 44, no. 10, p. 1065- 1072.
80. STOILOVA, I; KRASTANOV, Albert; STOYANOVA, A; DENEV, P; GARGOVA, S. Antioxidant activity of a ginger extract (Zingiber offici- nale). In: Food Chemistry. 2007. vol. 102, no. 3, p. 764–770.
81. VERMA, Ram S; VERMA, Rajesh K; PADALIA, Rajendra C; CHAUHAN, Amit; SINGH, Anand; SINGH, Hermendra P. Chemical Diversity in the Essential Oil of Indian Valerian (Valeriana jatamansi Jones). In: Chemis- try & Biodiversity. October, 2011. vol. 8, no. 10, p. 1921–1929.
82. WANG, Lei; GAO, Fan-sheng; XU, Kun; LI, Xiu. Natural occurrence of mixploid ginger (Zingiber officinale Rosc.) in China and its mor- phological variations. In: Scientia Horticulturae. June, 2014. vol. 172, p. 54–60.
83. WICAKSANA, Noladhi; GILANI, Syed Abdullah; AHMAD, Dawood; KIKUCHI, Akira; WATANABE, Kazuo N. Morphological and molecu- lar characterization of underutilized medicinal wild ginger ( Zingiber barbatum Wall.) from Myanmar. In: Plant Genetic Resources. Decem- ber, 2011. vol. 9, no. 4, p. 531–542.
84. WOHLMUTH, Hans; LEACH, David N; SMITH, Mike M; MYERS, Stephen P. Gingerol Content of Diploid and Tetraploid Clones of Gin- ger ( Zingiber officinale Roscoe). In: Journal Of Agricultural and Food Chemistry. June, 2005. vol. 53, no. 14, p. 5772–5778.
85. WOHLMUTH, Hans; SMITH, Mike M; BROOKS Lyndon O; MYERS, Sthepen P; LEACH, David N. Essential Oil Composition of Diploid and Tetraploid Clones of Ginger ( Zingiber officinale Roscoe ) Grown in Australia. In: Journal Of Agricultural and Food Chemistry. January, 2006. vol. 54, no. 4, p. 1414–1419.