Design of experiment to detect the level of probiotics in children's cereals in Molinos y piladoras Peter S.A.S. based on microbiological techniques
DOI:
https://doi.org/10.18041/1909-2458/ingeniare.26.6566Keywords:
Design of experiments, Probiotics, Microbiological TechniqueAbstract
In this article, the design proposed by the authors is a 2k experimental, evaluating the concentrations of probiotic microorganisms in infant cereals currently on the market. The tests were carried out in a Biotechnology laboratory where the interaction of the following factors was carried out: type of microorganism, temperature and type of planting, which were tested in a simulation in the digestive tract and methodology based on the type of planting direct and indirect in specific culture media with a count of colony forming units (CFU) for the identification of growth levels of Bifidobacterium Lactis and Bacillus Coagulans. In this practice, it is indicated that the probiotic "Bacillus Coagulans" is easy to detect in direct seeding; unlike "Bifidobacterium Lactis" which do not have a growth of lactic acid bacteria with any of the two seeds used, which means that there was resistance to the generation of colony forming units. It should be noted that the "Probiotic Microorganisms" in infant cereals must be present throughout the shelf life of the product and be easily released. For this reason, the factors that best intervene in CFUs are set at a temperature of 37ºC and based on an indirect methodology for obtaining lactic acid bacteria counts.
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