Bioinsecticide study for controlling the carrier ant (Atta cephalotes), using filamentous fungi spores Beauveria bassiana (ATCC MYA-4886) and Trichoderma lignorum (ATCC-8751)
DOI:
https://doi.org/10.18041/1900-3803/entramado.1.5417Keywords:
Fungi entomopathogenic, biological control, Leaf cutting ant, Beauvearia bassiana, richoderma lignorumAbstract
I. The Leaf cutting Ant is associated with losses in the agricultural sector, for the most part in the cultivation of citrus fruit sector, due to its activity defoliator. Control of the species has been handmade, chemical and biologically, the latter with environmental benefits and low risk to human health. This research had as objective develop a formulation biological for the control of the Leaf cutting Ant (Atta cephalotes) using a mixture of spores of two fungi filamentous (Beauveria bassiana and Trichoderma lignorum). M. He was the isolation of Beauveria bassiana (ATCC MYA-4886) and Trichoderma lignorum (ATCC 8751), through cultivation YPDA and was conducted identifying fungal imprint and biochemical tests. Developing five formulations with ratios of 1:1, 6:4, 4:6, 3:7, 2:8 of Beauveria bassiana and Trichoderma lignorum respectively, they underwent the test of viability in nutrient agar, pathogenicity by immersion test and proof of purity; the tests were performed in triplicate. R. The formulations presented viability to 24 h 95% 2, 100% of the formulations were pure after 10 days, formulations 6.4, 1:1, 2:8 infected all of the individuals in 6 days, formulations 4:6 and 3:7-8 days of exposure. C. Formulations 6.4, 1:1, 2:8 of Beauveria bassiana and Trichoderma lignorium, present infectious activity on the Leaf cutting Ant (Atta cephalotes) in laboratory.
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