Effect of disinfection treatments on explants of arracacha (Arracacia xanthorrhiza Bancroft)) for introduction in vitro
DOI:
https://doi.org/10.18041/1900-3803/entramado.1.10219Keywords:
Apiaceae, Biodiversity, Magnoliophyta, Root, PropagationAbstract
The arracacha is an Andean root with a high nutritional content, serving as an alternative source for food security. In Colombia, crop production has seen an increase due to the adoption of various genetic materials for commercial purposes across different regions of the country. However, the chosen method of propagation has led to a multiplication in the incidence of pests and diseases. The successful application of in vitro meristem propagation methods in multiplying Apiaceae plants prompted the objective of assessing the impact of different disinfection treatments and the introduction of arracacha explants in vitro. Two disinfection phases were carried out. In the initial phase, a completely randomized design (CRD) involved 14 treatments, assessing various concentrations and immersion durations of NaClO and OH. Subsequently, the treatment involving NaClO at 2%, yielding the best results in the first phase, was selected and combined with the application of bactericides such as chloramphenicol and gentamicin, each with different soaking times, resulting in 10 treatments. Effective disinfection of arracacha explants requires the use of 2% NaClO for 10 to 15 minutes, as this approach yields the lowest contamination percentages. Prolonged exposure of explants to NaClO resulted in higher percentages of cellular death, whereas the application of OH diminished tissue mortality. The inclusion of chloramphenicol reduced the contamination percentage of arracacha explants during in vitro multiplication.
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