Effect of pretreatment dilute acid -peroxide on napier grass (pennisetum purpureum schumach) to enhance reducing sugar yield by enzymatic hydrolysis
DOI:
https://doi.org/10.18041/1794-4953/avances.2.226Keywords:
Dilute acid, Hydrolysis, reducing sugars, napier, grass, Pennisetum purpureum, PeroxideAbstract
The napier grass (Pennisetum purpureum Schumach.) is a potential feedstock biomass for conversion to ethanol, bio-energy or high added value products. Variation in prehydrolysis parameters (time, acid strength, biomass %, particle size) and enzymatic saccharification conditions were examined for conversion of napier grass into fermentable sugars. A pretreatment of the water insoluble substrate (WISH2 SO4 ) at 15.1 % of reducing sugar yield (RSY) was done with peroxide (H2 02 ) at 122 °C. The relationship between RSY and the acid process parameters are described by a mathematical model derived from the experimental data.The peroxide pretreatment at 0.7 % (w/w), 4.75 mm particle size, 8% (w/v) biomass concentration, improved the RSY production from enzymatic hydrolysis in 97.6 % after 75 min. The enzymatic hydrolysis produced 287.81 mg/(g initial dry sample) of glucose and 245.81 mg/(g initial dry sample) of Xylose. Therefore, it was concluded that combination peroxide - acid pretreatment is an effective and environmentally friendly method for the enzyme hydrolysis of napier grass.
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