Características, métodos de cultivo y resistencia antimicrobiana en Helicobacter pylori
Palabras clave:
Helicobacter pylori, Crecimiento bacteriano, Medios de cultivo, Resistencia antibióticaResumen
Helicobacter pylori es la principal especie del genero Helicobacter responsable de una gran variedad de patologías gastroduodenales en países desarrollados y en vía de desarrollo. La importancia de cultivarla radica en la capacidad de conocer sus características de crecimiento, su diversidad genética, su epidemiologia, determinar la resistencia bacteriana a los antibióticos usados en determinado tratamiento y la posibilidad de realizar una detección exitosa en pacientes que se sospeche la presencia del microorganismo. Actualmente no se provee de un medio de cultivo universal para mantener el crecimiento de H. pylori y pocos medios han sido identificados que proveen significativamente el crecimiento ventajoso in vitro. Este artículo tiene como objetivo realizar una revisión de los diferentes medios de cultivo utilizados para el crecimiento in vitro de H. pylori, así como los mecanismos de resistencia de la bacteria
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Referencias
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Mobley, H.L.T., G.L. Mendz, and S.L. Hazell, in Helicobacter pylori: Physiology and Genetics, H.L.T. Mobley, G.L. Mendz, and S.L. Hazell, Editors. 2001: Washington (DC).
Banks, L., et al., Helicobacter Pylori, in Biological agents. 2012, International Agency for Research on Cancer: France.
Owen, R.J., Bacteriology of Helicobacter pylori. Baillieres Clin Gastroenterol, 1995. 9(3): p. 415-46.
Xia, H.X., et al., Transportation of Helicobacter pylori cultures by optimal systems. J Clin Microbiol, 1994. 32(12): p. 3075-7.
Cover, T.L., Perspectives on methodology for in vitro culture of Helicobacter pylori. Methods Mol Biol, 2012. 921: p. 11-5.
Hachem, C.Y., et al., Comparison of agar based media for primary isolation of Helicobacter pylori. J Clin Pathol, 1995. 48(8): p. 714-6.
Stevenson, T.H., et al., Growth of Helicobacter pylori in various liquid and plating media. Lett Appl Microbiol, 2000. 30(3): p. 192-6.
Westblom, T.U., E. Madan, and B.R. Midkiff, Egg yolk emulsion agar, a new medium for the cultivation of Helicobacter pylori. J Clin Microbiol, 1991. 29(4): p. 819-21.
Richards, C.L., et al., Optimizing the growth of stressed Helicobacter pylori. J Microbiol Methods, 2011. 84(2): p. 174-82.
Dent, J.C. and C.A. McNulty, Evaluation of a new selective medium for Campylobacter pylori. Eur J Clin Microbiol Infect Dis, 1988. 7(4): p. 555-8.
Henriksen, T.H., et al., Rapid growth of Helicobacter pylori. Eur J Clin Microbiol Infect Dis, 1995. 14(11): p. 1008-11. 70 MICROCIENCIA Investigación, Desarrollo e Innovación, Vol 7 – 2018
Kangatharalingam, N. and P.S. Amy, Helicobacter pylori comb. nov. Exhibits Facultative Acidophilism and Obligate Microaerophilism. Appl Environ Microbiol, 1994. 60(6): p. 2176-9.
Xu, J., S.J. Czinn, and T.G. Blanchard, Maintenance of Helicobacter pylori cultures in agar stabs. Helicobacter, 2010. 15(5): p. 477-80.
Testerman, T.L., et al., Nutritional requirements and antibiotic resistance patterns of Helicobacter species in chemically defined media. J Clin Microbiol, 2006. 44(5): p. 1650-8.
Coudron, P.E. and C.W. Stratton, Factors affecting growth and susceptibility testing of Helicobacter pylori in liquid media. J Clin Microbiol, 1995. 33(4): p. 1028-30.
Deshpande, M., E. Calenoff, and L. Daniels, Rapid large-scale growth of Helicobacter pylori in flasks and fermentors. Appl Environ Microbiol, 1995. 61(6): p. 2431-5.
Vega, A.E., et al., Growth of Helicobacter pylori in medium supplemented with cyanobacterial extract. J Clin Microbiol, 2003. 41(12): p. 5384-8.
Jiang, X. and M.P. Doyle, Growth supplements for Helicobacter pylori. J Clin Microbiol, 2000. 38(5): p. 1984-7.
Hutton, M.L., M. Kaparakis-Liaskos, and R.L. Ferrero, The use of AlbuMAX II((R)) as a blood or serum alternative for the culture of Helicobacter pylori. Helicobacter, 2012. 17(1): p. 68-76.
Joo, J.S., et al., A thin-layer liquid culture technique for the growth of Helicobacter pylori. Helicobacter, 2010. 15(4): p. 295-302.
Catrenich, C.E. and K.M. Makin, Characterization of the morphologic conversion of Helicobacter pylori from bacillary to coccoid forms. Scand J Gastroenterol Suppl, 1991. 181: p. 58- 64.
Dus, I., et al., Role of PCR in Helicobacter pylori diagnostics and research--new approaches for study of coccoid and spiral forms of the bacteria. Postepy Hig Med Dosw (Online), 2013. 67: p. 261-8.
Yamaguchi, H., et al., Colony formation by Helicobacter pylori after long-term incubation under anaerobic conditions. FEMS Microbiol Lett, 1999. 175(1): p. 107-11.
Siavoshi, F., et al., Mucoid Helicobacter pylori isolates with fast growth under microaerobic and aerobic conditions. Helicobacter, 2012. 17(1): p. 62-7.
Vega, A.E., H.J. Silva, and T.I. Cortinas, Evaluation of a serum-free transport medium supplemented with cyanobacterial extract, for the optimal survival of Helicobacter pylori from biopsy samples and strains. Eur J Clin Microbiol Infect Dis, 2012. 31(2): p. 135-9. 71 MICROCIENCIA Investigación, Desarrollo e Innovación, Vol 7 - 2018
Veenendaal, R.A., et al., Effect of transport medium and transportation time on culture of Helicobacter pylori from gastric biopsy specimens. J Clin Pathol, 1993. 46(6): p. 561-3.
Siu, L.K., et al., Evaluation of a selective transport medium for gastric biopsy specimens to be cultured for Helicobacter pylori. J Clin Microbiol, 1998. 36(10): p. 3048-50.
Cellini, L., et al., New transport medium for cultural recovery of Helicobacter pylori. J Clin Microbiol, 2014. 52(12): p. 4325-9.
Goodwin, C.S., et al., Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol, 1985. 38(10): p. 1127-31.
Krajden, S., et al., Comparison of selective and nonselective media for recovery of Campylobacter pylori from antral biopsies. J Clin Microbiol, 1987. 25(6): p. 1117-8.
Tee, W., et al., Comparative evaluation of three selective media and a nonselective medium for the culture of Helicobacter pylori from gastric biopsies. J Clin Microbiol, 1991. 29(11): p. 2587-9.
Olivieri, R., et al., Growth of Helicobacter pylori in media containing cyclodextrins. J Clin Microbiol, 1993. 31(1): p. 160-2.
Walsh, E.J. and A.P. Moran, Influence of medium composition on the growth and antigen expression of Helicobacter pylori. J Appl Microbiol, 1997. 83(1): p. 67-75.
Allahverdiyev, A.M., et al., Isolation and diagnosis of Helicobacter pylori by a new method: microcapillary culture. World J Gastroenterol, 2015. 21(9): p. 2622-8.
Ding, H.J., et al., An efficient method for the culture of Helicobacter pylori from gastric biopsies with two-section petri dishes. J Gastroenterol, 2001. 36(4): p. 237-41.
Kabir, S., Detection of Helicobacter pylori in faeces by culture, PCR and enzyme immunoassay. J Med Microbiol, 2001. 50(12): p. 1021-9.
Shahamat, M., et al., Evaluation of liquid media for growth of Helicobacter pylori. J Clin Microbiol, 1991. 29(12): p. 2835-7.
Morgan, D.R., et al., Growth of Campylobacter pylori in liquid media. J Clin Microbiol, 1987. 25(11): p. 2123-5.
Bessa, L.J., et al., Helicobacter pylori free-living and biofilm modes of growth: behavior in response to different culture media. APMIS, 2013. 121(6): p. 549-60.
Sainsus, N., et al., Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens. Eur J Clin Microbiol Infect Dis, 2008. 27(12): p. 72 MICROCIENCIA Investigación, Desarrollo e Innovación, Vol 7 - 2018 1209-17.
Young, K.A., et al., Quantitative culture of Helicobacter pylori from gastric juice: the potential for transmission. J Med Microbiol, 2000. 49(4): p. 343-7.
Megraud, F., Resistance of Helicobacter pylori to antibiotics. Alimentary pharmacology and Therapeutics, 1997. 11(1): p. 43-53.
Occhialini, A., et al., Macrolide resistance in Helicobacter pylori: rapid detection of point mutations and assays of macrolide binding to ribosomes. Antimicrob Agents Chemother, 1997. 41(12): p. 2724-8.
Gerrits, M.M., et al., Helicobacter pylori and antimicrobial resistance: molecular mechanisms and clinical implications. Lancet Infect Dis, 2006. 6(11): p. 699-709.
Aldana, L.P., et al., In vitro induction of resistance to metronidazole, and analysis of mutations in rdxA and frxA genes from Helicobacter pylori isolates. J Infect Chemother, 2005. 11(2): p. 59-63.
Marais, A., et al., Characterization of the genes rdxA and frxA involved in metronidazole resistance in Helicobacter pylori. Res Microbiol, 2003. 154(2): p. 137-44.
Vasquez, A., et al., Metronidazole and clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents in color indicator egg yolk agar in a miniwell format. J Clin Microbiol, 1996. 34(5): p. 1232-4.
Gerrits, M.M., et al., Alterations in penicillin-binding protein 1A confer resistance to beta-lactam antibiotics in Helicobacter pylori. Antimicrob Agents Chemother, 2002. 46(7): p. 2229-33
Paul, R., et al., Mutations of the Helicobacter pylori genes rdxA and pbp1 cause resistance against metronidazole and amoxicillin. Antimicrob Agents Chemother, 2001. 45(3): p. 962-5.
Mobley, H.L.T., G.L. Mendz, and S.L. Hazell, in Helicobacter pylori: Physiology and Genetics, H.L.T. Mobley, G.L. Mendz, and S.L. Hazell, Editors. 2001: Washington (DC).
Banks, L., et al., Helicobacter Pylori, in Biological agents. 2012, International Agency for Research on Cancer: France.
Owen, R.J., Bacteriology of Helicobacter pylori. Baillieres Clin Gastroenterol, 1995. 9(3): p. 415-46.
Xia, H.X., et al., Transportation of Helicobacter pylori cultures by optimal systems. J Clin Microbiol, 1994. 32(12): p. 3075-7.
Cover, T.L., Perspectives on methodology for in vitro culture of Helicobacter pylori. Methods Mol Biol, 2012. 921: p. 11-5.
Hachem, C.Y., et al., Comparison of agar based media for primary isolation of Helicobacter pylori. J Clin Pathol, 1995. 48(8): p. 714-6.
Stevenson, T.H., et al., Growth of Helicobacter pylori in various liquid and plating media. Lett Appl Microbiol, 2000. 30(3): p. 192-6.
Westblom, T.U., E. Madan, and B.R. Midkiff, Egg yolk emulsion agar, a new medium for the cultivation of Helicobacter pylori. J Clin Microbiol, 1991. 29(4): p. 819-21.
Richards, C.L., et al., Optimizing the growth of stressed Helicobacter pylori. J Microbiol Methods, 2011. 84(2): p. 174-82.
Dent, J.C. and C.A. McNulty, Evaluation of a new selective medium for Campylobacter pylori. Eur J Clin Microbiol Infect Dis, 1988. 7(4): p. 555-8.
Henriksen, T.H., et al., Rapid growth of Helicobacter pylori. Eur J Clin Microbiol Infect Dis, 1995. 14(11): p. 1008-11. 70 MICROCIENCIA Investigación, Desarrollo e Innovación, Vol 7 – 2018
Kangatharalingam, N. and P.S. Amy, Helicobacter pylori comb. nov. Exhibits Facultative Acidophilism and Obligate Microaerophilism. Appl Environ Microbiol, 1994. 60(6): p. 2176-9.
Xu, J., S.J. Czinn, and T.G. Blanchard, Maintenance of Helicobacter pylori cultures in agar stabs. Helicobacter, 2010. 15(5): p. 477-80.
Testerman, T.L., et al., Nutritional requirements and antibiotic resistance patterns of Helicobacter species in chemically defined media. J Clin Microbiol, 2006. 44(5): p. 1650-8.
Coudron, P.E. and C.W. Stratton, Factors affecting growth and susceptibility testing of Helicobacter pylori in liquid media. J Clin Microbiol, 1995. 33(4): p. 1028-30.
Deshpande, M., E. Calenoff, and L. Daniels, Rapid large-scale growth of Helicobacter pylori in flasks and fermentors. Appl Environ Microbiol, 1995. 61(6): p. 2431-5.
Vega, A.E., et al., Growth of Helicobacter pylori in medium supplemented with cyanobacterial extract. J Clin Microbiol, 2003. 41(12): p. 5384-8.
Jiang, X. and M.P. Doyle, Growth supplements for Helicobacter pylori. J Clin Microbiol, 2000. 38(5): p. 1984-7.
Hutton, M.L., M. Kaparakis-Liaskos, and R.L. Ferrero, The use of AlbuMAX II((R)) as a blood or serum alternative for the culture of Helicobacter pylori. Helicobacter, 2012. 17(1): p. 68-76.
Joo, J.S., et al., A thin-layer liquid culture technique for the growth of Helicobacter pylori. Helicobacter, 2010. 15(4): p. 295-302.
Catrenich, C.E. and K.M. Makin, Characterization of the morphologic conversion of Helicobacter pylori from bacillary to coccoid forms. Scand J Gastroenterol Suppl, 1991. 181: p. 58- 64.
Dus, I., et al., Role of PCR in Helicobacter pylori diagnostics and research--new approaches for study of coccoid and spiral forms of the bacteria. Postepy Hig Med Dosw (Online), 2013. 67: p. 261-8.
Yamaguchi, H., et al., Colony formation by Helicobacter pylori after long-term incubation under anaerobic conditions. FEMS Microbiol Lett, 1999. 175(1): p. 107-11.
Siavoshi, F., et al., Mucoid Helicobacter pylori isolates with fast growth under microaerobic and aerobic conditions. Helicobacter, 2012. 17(1): p. 62-7.
Vega, A.E., H.J. Silva, and T.I. Cortinas, Evaluation of a serum-free transport medium supplemented with cyanobacterial extract, for the optimal survival of Helicobacter pylori from biopsy samples and strains. Eur J Clin Microbiol Infect Dis, 2012. 31(2): p. 135-9. 71 MICROCIENCIA Investigación, Desarrollo e Innovación, Vol 7 - 2018
Veenendaal, R.A., et al., Effect of transport medium and transportation time on culture of Helicobacter pylori from gastric biopsy specimens. J Clin Pathol, 1993. 46(6): p. 561-3.
Siu, L.K., et al., Evaluation of a selective transport medium for gastric biopsy specimens to be cultured for Helicobacter pylori. J Clin Microbiol, 1998. 36(10): p. 3048-50.
Cellini, L., et al., New transport medium for cultural recovery of Helicobacter pylori. J Clin Microbiol, 2014. 52(12): p. 4325-9.
Goodwin, C.S., et al., Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol, 1985. 38(10): p. 1127-31.
Krajden, S., et al., Comparison of selective and nonselective media for recovery of Campylobacter pylori from antral biopsies. J Clin Microbiol, 1987. 25(6): p. 1117-8.
Tee, W., et al., Comparative evaluation of three selective media and a nonselective medium for the culture of Helicobacter pylori from gastric biopsies. J Clin Microbiol, 1991. 29(11): p. 2587-9.
Olivieri, R., et al., Growth of Helicobacter pylori in media containing cyclodextrins. J Clin Microbiol, 1993. 31(1): p. 160-2.
Walsh, E.J. and A.P. Moran, Influence of medium composition on the growth and antigen expression of Helicobacter pylori. J Appl Microbiol, 1997. 83(1): p. 67-75.
Allahverdiyev, A.M., et al., Isolation and diagnosis of Helicobacter pylori by a new method: microcapillary culture. World J Gastroenterol, 2015. 21(9): p. 2622-8.
Ding, H.J., et al., An efficient method for the culture of Helicobacter pylori from gastric biopsies with two-section petri dishes. J Gastroenterol, 2001. 36(4): p. 237-41.
Kabir, S., Detection of Helicobacter pylori in faeces by culture, PCR and enzyme immunoassay. J Med Microbiol, 2001. 50(12): p. 1021-9.
Shahamat, M., et al., Evaluation of liquid media for growth of Helicobacter pylori. J Clin Microbiol, 1991. 29(12): p. 2835-7.
Morgan, D.R., et al., Growth of Campylobacter pylori in liquid media. J Clin Microbiol, 1987. 25(11): p. 2123-5.
Bessa, L.J., et al., Helicobacter pylori free-living and biofilm modes of growth: behavior in response to different culture media. APMIS, 2013. 121(6): p. 549-60.
Sainsus, N., et al., Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens. Eur J Clin Microbiol Infect Dis, 2008. 27(12): p. 72 MICROCIENCIA Investigación, Desarrollo e Innovación, Vol 7 - 2018 1209-17.
Young, K.A., et al., Quantitative culture of Helicobacter pylori from gastric juice: the potential for transmission. J Med Microbiol, 2000. 49(4): p. 343-7.
Megraud, F., Resistance of Helicobacter pylori to antibiotics. Alimentary pharmacology and Therapeutics, 1997. 11(1): p. 43-53.
Occhialini, A., et al., Macrolide resistance in Helicobacter pylori: rapid detection of point mutations and assays of macrolide binding to ribosomes. Antimicrob Agents Chemother, 1997. 41(12): p. 2724-8.
Gerrits, M.M., et al., Helicobacter pylori and antimicrobial resistance: molecular mechanisms and clinical implications. Lancet Infect Dis, 2006. 6(11): p. 699-709.
Aldana, L.P., et al., In vitro induction of resistance to metronidazole, and analysis of mutations in rdxA and frxA genes from Helicobacter pylori isolates. J Infect Chemother, 2005. 11(2): p. 59-63.
Marais, A., et al., Characterization of the genes rdxA and frxA involved in metronidazole resistance in Helicobacter pylori. Res Microbiol, 2003. 154(2): p. 137-44.
Vasquez, A., et al., Metronidazole and clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents in color indicator egg yolk agar in a miniwell format. J Clin Microbiol, 1996. 34(5): p. 1232-4.
Gerrits, M.M., et al., Alterations in penicillin-binding protein 1A confer resistance to beta-lactam antibiotics in Helicobacter pylori. Antimicrob Agents Chemother, 2002. 46(7): p. 2229-33
Paul, R., et al., Mutations of the Helicobacter pylori genes rdxA and pbp1 cause resistance against metronidazole and amoxicillin. Antimicrob Agents Chemother, 2001. 45(3): p. 962-5.
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2019-10-07
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Cardona, J., B., M. A, C. L., & Aldana A, Álvarez. (2019). Características, métodos de cultivo y resistencia antimicrobiana en Helicobacter pylori. Microciencia, 7, 56-72. https://revistas.unilibre.edu.co/index.php/microciencia/article/view/5618
