IDENTIFICATION OF HELICOBACTER PYLORI FROM HUMAN CORONARY ATEROSCLERÓTICA INJURY
DOI:
https://doi.org/10.18041/2390-0512/biociencias.1.5321Keywords:
Atherosclerotic disease, atherosclerosis, inflammation, infection, Helicobacter pyloriAbstract
Introduction: The main pathogenic mechanism underlying the genesis and development of diseases of the circulatory system is atherosclerosis. It is a complex and progressive pathological process of the arterial wall that affects especially the coronary, cerebral and peripheral arteries. Currently, there is talk about "The infectious hypothesis of atherosclerosis". Helicobacter pylori infection has been one of the most researched worldwide. Objective: To identify H. pylori from atherosclerotic lesions of patients who attend the cardiovascular and angiology services of the city of Barranquilla. Methods: A descriptive cross-sectional study was carried out. In a sample of 102 participants. The atheromas were taken by expert personnel in endarterectomy and dissection of blood vessels. The specimens were embedded in 4% formaldehyde solution. DNA extraction was performed from atheromas. The molecular diagnosis of H. pylori was performed by ANIDADA PCR, evidencing a 120 bp fragment after electrophoresis in a 3.5% agarose gel in 0.5X TBE. Results: 102 samples of atheromas were obtained. One sample was positive for H. pylori (Sample # 14, 1/102). The atherosclerotic tissue was obtained from the right coronary artery. Conclusion: The infectious hypothesis of atherosclerosis has been one of the main research topics worldwide in recent decades. H. pylori infection is a risk factor, however, several studies are necessary to be able to conclude more accurately.
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References
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2. Indicadores Básicos Situación de Salud en Colombia 2011. Ministerio de salud y protección social. https://www.minsalud.gov.co/salud/.../indicadoresbasicossp.aspx
3. Análisis de situación de salud del distrito de barranquilla año 2016. www.barranquilla.gov.co/salud/index.php/informes/doc_download/198-
4. Libby P, Bornfeldt KE, Tall AR. Atherosclerosis: successes, surprises, and future challenges. Circ Res 2016; 118:531-4.
5. Tarkin JM, Dweck MR, Evans NR, Takx RAP, Brown AJ, Tawakol A, Fayad ZA, Rudd JHF. Imaging atherosclerosis. Circ Res. 2016; 118:750 –769. doi: 10.1161/CIRCRESAHA.115.306247.
6. Gimbrone MA Jr, Garcia-Cardena G. Endothelial cell dysfunction and the pathobiology of atherosclerosis. Circ Res. 2016; 118:620–636. doi:10.1161/CIRCRESAHA.115.306301.
7. R. Ross, Atherosclerosis an inflammatory disease, N. Engl. J. Med. 340 (1999)115-126.
8. P. Libby, G. Pasterkamp, Requiem for the 'vulnerable plaque', Eur. Heart J. 36(43) (2015 Nov 14) 2984-2987, https://doi.org/10.1093/eurheartj/ehv349.
9. Mozaffarian D, Benjamin EJ, Go AS, et al. Heart disease and stroke statistics — 2016 update: a report from the American Heart Association. Circulation 2016;133(4): e38-e360.
10. E. Falk, M. Nakano, J.F. Bentzon, et al., Update on acute coronary syndromes: the pathologists' view, Eur. Heart J. 34 (2013) 719-728.
11. Anderson, J. L., and Morrow, D.A., Acute Myocardial Infarction. N Engl J Med. 2017; 376:2053-64. DOI: 10.1056/NEJMra1606915.
12. Langhorne P, Fearon P, Ronning OM, Kaste M, Palomaki H, Vemmos K, et al. Stroke unit care benefits patients with intracerebral hemorrhage: systematic review and meta-analysis. Stroke. 2013;44(11):3044-9.
13. Laslett LJ, Alagona P Jr, Clark BA, Drozda JP Jr, Saldivar F, Wilson SR, et al. The worldwide environment of cardiovascular disease: prevalence, diagnosis, therapy, and policy issues: a report from the American College of Cardiology. J Am Coll Cardiol. 2012;60:S1-S49.
14. Tsivgoulis G, Safouris A, Kim DE, and Alexandro AV. Recent Advances in Primary and Secondary Prevention of Atherosclerotic Stroke. J Stroke. 2018 May; 20(2): 145–166. doi: [10.5853/jos.2018.00773]
15. Campbell LA and Rosenfeld ME. Infection and Atherosclerosis Development. Arch Med Res. 2015 Jul; 46(5): 339–350. doi:[10.1016/j.arcmed.2015.05.006]
16. Pothineni NVK, Subramany S, Kuriakose K, Shirazi LF, Romeo F, Shah PK, Mehta JL. Infections, atherosclerosis, and coronary heart disease.Eur Heart J. 2017;38:3195–3201. doi: 10.1093/eurheartj/ehx362
17. Shana J, Baib X, Han L, Yuan Y, Yang J and Sun X. Association between atherosclerosis and gastric biomarkers concerning Helicobacter pylori infection in a Chinese healthy population. Experimental Gerontology 2018;112: 97–102.
18. Kountouras J, Polyzos SA, Katsinelos P, Zeglinas C, Artemaki F, Tzivras D, et al. Cardio-cerebrovascular disease and Helicobacter pylori-related metabolic syndrome: We consider eradication therapy as a potential cardio-cerebrovascular prevention strategy. Int J Cardiol. 2017; 229: 17–18. https://doi.org/10.1016/j.ijcard.2016.11.265 PMID: 27887801
19. Sun J, Rangan P, Bhat SS, Liu L. A Meta-Analysis of the Association between Helicobacter pylori Infection and Risk of Coronary Heart Disease from Published Prospective Studies. Helicobacter. 2016; 21:11–23. https://doi.org/10.1111/hel.12234 PMID: 25997465
20. García A, Contreras, J. 2010. Estandarización de una técnica de PCR anidada para la detección de Helicobacter pylori. Tesis de grado Universidad de Pamplona.
21. Blaser MJ, Atherton JC. Helicobacter pylori persistence: biology and disease. JCI. 2004; 113:321-33.
22. Goodman KJ, Joyce SL, Ismond KP. Extragastric diseases associated with Helicobacter pylori infection. Current Gastroenterology Reports. 2006; 8:458-64.
23. Budzynski J, Kozinski M, Klopocka M et al. Clinical signifi cance of Helicobacter pylori infection in patients with acute coronary syndromes: an overview of current evidence. Clin Res Cardiol 2014; 103:855 – 86.
24. Coskun S, Kasirga E, Yilmaz O, Bayindir P, Akil I, Yuksel H, Polat M, Sanlidag T. Is Helicobacter pylori related to endothelial dysfunction during childhood? Pediatr Int 2008; 50: 150-153 [PMID:18353048 DOI: 10.1111/j.1442-200X.2008.02542.x]
25. Ayada K, Yokota K, Kobayashi K, Shoenfeld Y, Matsuura E, Oguma K. Chronic infections and atherosclerosis. Ann N Y Acad Sci. 2007; 1108: 594±602. PMID: 17894024
26. Pellicano R, Mazzarello MG, Morelloni S, Allegri M, Arena V, Ferrari M, Rizzetto M, Ponzetto A. Acute myocardial infarction and Helicobacter pylori seropositivity. Int J Clin Lab Res 1999; 29:141-144 [PMID: 10784374 DOI: 10.1007/s005990050080]
27. Vijayvergiya R, Agarwal N, Bahl A, Grover A, Singh M, Sharma M, Khullar M. Association of Chlamydia pneumoniae and Helicobacter pylori infection with angiographically demonstrated coronary artery disease. Int J Cardiol 2006; 107: 428-429 [PMID: 16503271 DOI:10.1016/j.ijcard.2005.02.028]
28. Franceschi F, Niccoli G, Ferrante G, Gasbarrini A, Baldi A, Candelli M, Feroce F, Saulnier N, Conte M, Roccarina D, Lanza GA, Gasbarrini G, Gentiloni SN, Crea F. CagA antigen of Helicobacter pylori and coronary instability: insight from a clinico-pathological study and a meta-analysis of 4241 cases. Atherosclerosis 2009; 202:535-542 [PMID: 18599062 DOI: 10.1016/j.atherosclerosis.2008.04.051]
29. Goyal P, Kalek SC, Chaudhry R, Chauhan S, Shah N. Association of common chronic infections with coronary artery disease in patients without any conventional risk factors. Indian J Med Res 2007; 125: 129-136 [PMID: 17431281]
30. Assadi M, Saghari M, Ebrahimi A, Reza Pourbehi M, Eftekhari M, Nabipour I, Abbaszadeh M, Nazarahari M, Nasiri M, Assadi S. The relation between Helicobacter pylori infection and cardiac síndrome X: a preliminary study. Int J Cardiol 2009; 134: e124-e125 [PMID: 18501447 DOI: 10.1016/j.ijcard.2008.01.029]
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2019-04-26
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