BACs obtaining bases on pseudorabies virus without packaging sequences by homologous recombination in E. coli
Keywords:
Amplicons, Pseudorabies, Homologous recombination, Pac sequencesAbstract
The herpes simplex virus-based vectors have great advantages such as being neurotropic, efficient infection, es- tablishment of latency, capacity to transport large amounts of DNA, but also have a major drawback being a pathogenic virus for humans, even undergoing modification to alter its virulence it can never be ruled out. Pack- aging sequences (pac) are necessary for correct cutting and packaging of the virus and are located in BamHI fragments 13 and 14’ respectively with a size of 3.2 Kpb. The objective of the study was to obtain BACs based on pseudorabies virus without packaging sequences (pac). PRV BACs modifying was made using homologous recom- bination into DH10B cells and recombination was verified by hybridization analysis.
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References
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11.Enquist LW, Husak PJ,. Banfield BW, Smith GA. Infection and spread of alphaherpesvi- ruses in nervous system. AdvVirus Reearch. 1998; 51:237-347.
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13.Winkeler A, Sena-Esteves M, Paulis LE, Li H, Waerzeggers Y, Ruckriem B, et al. Switching on the lights for gene therapy. Plos One. 2007; 2:e528.
Kwong AD, Frenkel N. Herpes simplex virus amplicon: effect of size on replication of constructed defective genome containing eukaryotic DNA sequences. J. Virol. 1984; 51:595-603.
3.Fraefel C, Jacoby DR, Braekefield XO. Herpes simplex virus type 1-based amplicon vector systems. Adv Virus Res. 2000; 55:425-51.
4.Saeki Y, Breakefield XO, Chiocen EA. Impro- ved HSV-1 amplicon packaging system using ICP27-deleted, oversized HSV-1 BAC DNA. Methods. Mol Med. 2003; 76:51-60.
5.Spaete R, Frenkel N. The herpes simplex vi- rus amplicón: a new eucaryotic defective virus cloning-amplifying vector. Cell. 1982; 30(1):295-304.
6.Saeki Y, Ichikawa T, Saeki A, Chiocca EA, To- bler K, Ackermann M, et al. Herpes simplex virus type 1 DNA amplified as bacterial arti- ficial chromosome in Escherichia coli: rescue of replication-competent virus progeny and packaging of amplicon vectors. Hum Gen Ther. 1998; 9:2787-94.
7.Smith GA, Enquist LW. A self-recombining bacterial artificial chromosome and its appli- cation for analysis of herpes virus pathoge- nesis. Proc. Natl. Acad. 2000; 97:4873-8.
8.Narayanan K, Williamson R, Zhang Y, Stewart AF, Ioannou PA. Efficient and precise engi- neering of a 200 Kb B-globin human/bacte- rial artificial chromosome in E. coli DH10B using an inducible homologous recombina- tion system. Gene Therapy. 1999; 6:442-7.
9.Sambrook J, Russell DW. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Har- bor Laboratory Press, Cold Spring Harbor, N. Y.; 2001.
10.Tabares E. Detetion of DNA viruses by radioactive DNA probes: application to African swine fever virus. Arch Virol. 1987; 92:233-42.
11.Enquist LW, Husak PJ,. Banfield BW, Smith GA. Infection and spread of alphaherpesvi- ruses in nervous system. AdvVirus Reearch. 1998; 51:237-347.
12.Tyler CM, Wuertzer CA, Bowers WJ, Fede- roff HJ. HSV amplicones: Neuro applications. Curr. Gene Ther. 2006; 6:337-50.
13.Winkeler A, Sena-Esteves M, Paulis LE, Li H, Waerzeggers Y, Ruckriem B, et al. Switching on the lights for gene therapy. Plos One. 2007; 2:e528.
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Published
2012-12-01
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